Composite

Part:BBa_K2598045:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-10-04)


amilCP+fwyellow


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1752


Design Notes

The same genetic contexts are constructed to enssure 1:1 expression, Double terminators are added to avoid leakage.



Source

The subparts are proivded by Lou lab.

References